Crossflow microfiltration of recombinantEscherichia coli lysates after high pressure homogenization

Crossflow microfiltration of recombinant Escherichia coli lysates after high pressure homogenization.

Crossflow membrane filtration was used to process recombinant Escherichia coli cell lysates containing protein inclusion bodies after high pressure homogenization. The number of passes through the high pressure homogenizer changed the viscosities and average particle sizes of the cell lysates. The different cell lysates were processed with a hollow fiber unit containing microfiltration membrane...

Crossflow filtration for CHO cell separation by microfiltration using crossflow systems.

Chinese Hamster Ovary (CHO) cells are used for fermentation of high value proteins in the pharmaceutical- and biotechnology industry. During the fermentation process the cells are grown under specific conditions in a defined media. The CHO cells produce extra cellular proteins. Separation and purification of these proteins result in the final product of high value. The production steps utilized...

Sinusoidal crossflow microfiltration device--experimental and computational flowfield analysis.

We present an analysis of the flowfield inside a novel crossflow microfiltration device. The filter performance relies on shear focusing by means of a corrugated channel. The flow and shear stress characteristics inside the filter are studied by means of both micro Particle Image Velocimetry (micro-PIV) measurements and Computational Fluid Dynamics (CFD) analysis. We show that an increase of th...

Microbial Cell Disruption by High-Pressure Homogenization

Production of poly-b-hydroxybutyrateon molasses by recombinantEscherichia coli

Beet molasses successfully replaced glucose as sole carbon source to produce poly-b-hydroxybutyrate by a recombinant Escherichia coli strain (HMS174/pTZ18u-PHB). The fermentation with molasses was cheaper than with glucose. The final dry cell weight, PHB content and PHB productivity were 39.5 g/L, 80% (w/w) and 1 g/Lh, respectively, in a 5 L stirred tank fermenter after 31.5 h fed-batch ferment...